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Marker-assisted backcrossing using microsatellites and validation of SCAR Phs marker for resistance to white mold in common bean Electron. J. Biotechnol.
Carneiro,Flávia Fernandes; Santos,João Bosco dos; Leite,Monik Evelin.
Molecular markers may accelerate selection through the identification of plants with higher proportion of recurrent parent genome, as well as identifying those plants bearing target alleles like quantitative traits loci (QTLs) for white mold resistance. The objectives of this work were: 1) to employ microsatellite markers (SSR) in order to accelerate the recovery of recurrent parent genome 2) to validate sequence characterized amplified region (SCAR) Phs associated with a QTL that confers resistance to white mold, as previously identified in bean populations. Lines G122 and M20 were crossed, which generated 267 F1 plants from backcross (BC) BC1 and 113 plants from backcross BC2.SSR polymorphic markers were adopted. The relationship between BC plants and...
Tipo: Journal article Palavras-chave: Backcross; Genetic similarity; Phaseolus vulgaris; SCAR; Sclerotinia sclerotiorum; SSR assisted selection.
Ano: 2010 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000600009
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Natural selection in common bean microsatellite alleles and identification of QTLs for grain yield Electron. J. Biotechnol.
Leite,Monik Evelin; Santos,João Bosco dos; Carneiro,Flávia Fernandes; Couto,Karla Rodrigues.
Natural selection acts to select better adapted individuals or alleles in segregating population and help plant breeding. The objective of this work was to verify the effect of natural selection on microsatellite alleles as indicators of better adaptation and identification of quantitative trait loci (QTLs) for grain yield. This study evaluated 107 progenies from the F8 and 107 from the F24 generation derived from crossing Carioca MG and ESAL 686 lines, carried out by the bulk method, and evaluated in three different seasons: winter 2001; rainy 2001 and dry 2002. It was utilized 22 polymorphic markers and the natural selection acted in all of them. The frequency of the alleles of the parent Carioca MG, the most adapted, was increased in all of the 22 loci...
Tipo: Journal article Palavras-chave: Bulk method; Fitness coefficient; Phaseolus vulgaris; SSR markers.
Ano: 2011 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582011000100005
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